Synthesis and Evaluation of 8-halogenated-7-deaza-2 ́-deoxy-guanosine as 8-oxo-2 ́-deoxy-guanosine analogues
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چکیده
【Introduction】 8-Oxo-2 ́-deoxyguanosine (8-oxo-dG) is a representative nucleoside damage that is formed by oxidation of 2 ́-deoxyguanosine (dG) with reactive oxygen species (ROS), and its presence has been linked to aging, cancer, etc [1]. Unlike dG, 8-oxo-dG forms stable base pairs with both 2 ́-deoxycytidine (dC) and 2 ́-deoxyadenosine (dA). Based on the base-pairing properties of 8-oxo-dG, DNA polymerases incorporate 8-oxo-dGTP opposite dA and dATP opposite 8-oxo-dG, causing AT to CG and GC to TA transversion mutations. To suppress the genotoxicity of 8-oxo-dG and protect the genome integrity, hOGG1 can excise 8-oxo-dG from 8-oxo-dG:dC base pairs within duplex DNA. And hMYH provides the defense by removing dA opposite 8-oxo-dG. To prevent the incorporation
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